1H-tetrazolium acetate - Names and Identifiers
1H-tetrazolium acetate - Physico-chemical Properties
| Molecular Formula | C3H6N4O2
|
| Molar Mass | 130.10534 |
1H-tetrazolium acetate - Introduction
1H-tetrazolium acetate, also known as 1H-tetrazolium acetate, is a chemical reagent commonly used for staining in biology and histology. The following is a description of the nature, use, preparation and safety information of 1H-tetrazolium acetate:
Nature:
1H-tetrazolium acetate is a red crystalline powder that can be reduced to a colorless solution in an acidic environment. It has high water solubility and can also be dissolved in alcohol.
Use:
1H-tetrazolium acetate is mainly used to determine the viability and vitality of cells. It can generate a purple product through the reduction reaction, and react with the electron transfer body in the cell to form a visible spectrum absorption peak. In cell biology and histology, 1H-tetrazolium acetate is commonly used as a stain to evaluate cell viability, respiratory function and metabolic activity. In addition, it is also applied to the detection and identification of bacteria and fungi.
Method:
The preparation method of 1H-tetrazolium acetate is relatively simple, and it is generally obtained by benzene nitration and acylation. The specific preparation method is as follows: first, Aniline reacts with nitric acid in the presence of sulfuric acid to produce benzenitramine; Then, benzenitramine and ammonia water are acylated with acetic acid to obtain 1H-tetrazolium acetate.
Safety Information:
When using 1H-tetrazolium acetate, pay attention to safety and avoid contact with skin, eyes and respiratory tract. It belongs to a toxic chemical, which may cause irritation and harm to the human body. During use, it is recommended to wear appropriate protective equipment, such as gloves, masks and goggles. Also, avoid storage and use with oxidants and combustibles. If you accidentally touch, swallow, or inhale the chemical, seek medical attention immediately. In order to ensure safety, the relevant safety instructions should be read and followed carefully before use.
Last Update：2024-04-09 21:01:54